online course on
Real time monitoring of sea contaminants by an autonomous lab-on-a-chip biosensor

This course is designed to familiarise aquaculture farmers, researchers, and other interested stakeholders with the SEA-on-a-CHIP device, a miniaturized, autonomous, remote and flexible immuno-sensor platform for real-time analysis of marine waters in estuarine and coastal areas, in multi-stressor conditions.

Description

Chemical contamination of estuarine and coastal areas is a highly complex issue with negative implications for the environment and human health (through the food chain) and related coastal industries such as fisheries. The main goal of the EU-funded project SEA-on-a-CHIP was to develop a compact, miniaturized and autonomous on-site analytical system for real-time monitoring of harmful contaminants in seawater, developed to be used as an early-warning system in aquaculture facilities.

The device provides an extremely sensitive and selective detection of the target compounds through an indirect competitive immunoassay combined with electrochemical detection. A floating outer casing hide the Lab-on-a-Chip system integrated into a highly engineering sampling and microfluidic unit, together with communication module which manage the whole analytical procedure and data transmission. The power supply and liquid reservoirs allow the device working autonomously for one month making two daily seawater sampling and simultaneous analysis of a set of 7 representative contaminants and one control. The units have been tested throughout the lifetime of the project and calibrated to state-of-the-art of chemical analytics.

The aim of this online course is to provide a general overview of monitoring sea contaminants, and to showcase the SEA-on-a-CHIP device, its characteristics, its potential, and the challenges for its application in aquaculture farms. The target of the course are students and researchers, and aquaculture farmers and associations. Also policy makers and environmental protection agencies may find useful and interesting information about the most recent developments in the field of early-warning systems for real-time analysis of marine waters - in estuarine and coastal areas - in multi-stressor conditions.

Presentations
Lessons
ProfessorChaptersTitles of the lessonsMaterials
Dr. Marinella Farré
Dr. Marinella Farré

She has a Ph.D. in Chemistry from University of Barcelona (Dec.2003), and she is Research scientist, permanent staff at IDÆA-CSIC from Jul. 2008. She has 140 scientific papers in journals of SCI (H-index of 30), 20 book chapters and she is co-Editor of a book. She has supervised 3 PhD Thesis and currently is the supervisor of 4 on-going PhD Thesis. She is involved in development of biosensors based on new nano-technological materials, and development, application of new techniques of screening for the analysis of micro organic pollutants in environmental matrices with chemical analysis. She was involved in different EU projects.


1
The SEA-on-a-CHIP project
The SEA-on-a-CHIP project

The presentation briefly introduces coastal ecosystem services and gives an overview of the EU-funded SEA-on-a-CHIP project.

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Assoc. Prof. Martin Hedström
Assoc. Prof. Martin Hedström

Martin Hedström is COO of CapSenze, and holds an associate professorship at Lund University. He obtained his PhD in biotechnology in year 2006, specializing in bioanalytical chemistry and biosensor technologies. He is founding partner in CapSenze, building the company around his main research topic regarding the flow-based bioanalytical technologies. He is still active at Lund University as head of the bioanalysis group at the Department of Biotechnology, where he supervises PhD students.

2
SEA-on-a-CHIP device
SEA-on-a-CHIP device

The presentation describes the overview of the SEA-on-a-CHIP device with emphasis on the integration of the different subunits, external- and internal housing and the general device functions related to e.g. floatability, main (high level) workflow of the assay and maintenance. Some detailed descriptions regarding liquid handling are power supply are involved.

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Dr. Juan Pablo Salvador
Dr. Juan Pablo Salvador

I am a contracted researcher from CIBER-BBN, adscript to the Nb4D group headed by Prof. M.-Pilar Marco at IQAC-CSIC. My expertise is immunochemistry that involves several disciplines such as organic and analytical chemistry, surface chemistry and knowledge in some optical and electrochemical properties. I have published more than 20 publications in a peer review journals and co-author in 2 patents, one of them developed in the context of SEA-on-a-CHIP project. I am responsible for different national and European projects.

3
Immunoassay
Immunoassay

This presentation summarises the main achievements occurred during the SEA-on-a-CHIP project from the immunoassay development point of view. Basically, the immunoassay module consisted in providing the immunoreagents that would allow the specific recognition of the targeted pollutants in the SEA-on-a-CHIP device. The biomolecules responsible for the specific recognition of the targeted pollutants are the antibodies which will be detected by the immunosensor (detection module).

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Video
Dr. Josep Riera
Dr. Josep Riera

CEO of the enterprise Organización, Calidad y Proyectos, S.L. which sells consultancy as main activity. We are devoted to serve what the client needs to implement projects about organization of his premises and those projects include implementing new software either standard packages or customized ones. The enterprise has developed several product lines of standard software ranging from web shops to on-line databases to store data generate by devices of several types.

4
Remote control and user software
Remote control and user software

This presentation focuses on the remote-control software, which is a set of several elements that allow to pick up data from a widen network of biosensors and put them into a database through the internet connection. It’s especially important in this case because the biosensor is off shore, in the sea and the only knowledge about his performance is data which send to this database. This software has been designed to fulfil the requirements of the project but it can be connected to other kind of sensors.

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Prof.James Readman
Prof.James Readman

Professor Readman leads fundamental research into the composition/cycling of organic material and the behaviour and impact of pollutants in estuarine and coastal environments. He has gained professional experience in academic, Governmental, industrial contract research and United Nations laboratories, including seven years employment by the UN as Head of the Organic Chemistry Unit at the IAEA-MEL based in Monaco. He has published over 120 peer-reviewed papers which generate an h-index of 44.

5
Verification and validation
Verification and validation

This presentation focuses on the verification and validations processes of the SEA-on-a-CHIP device. It briefly explains how laboratory studies, mesocosm studies and field studies were carried out for this purpose.

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Certificate

To obtain a certification of attendance you have to complete all modules included in the course watching all materials and answering correctly to the relative quiz included. For more information contact the tutor of the course (Dr. Alice Tediosi).

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